Article
Double immunohistochemical detection of transmitter phenotype of proliferating cells using bromodeoxyuridine.
Neuroscience letters - 28 Oct 1991
Kono T, Takada M, Wu J Y, Kitai S T
Abstract excerpt
We describe a new method that can determine transmitter phenotype of proliferating nerve cells at a given age. The procedure is based on indirect sequential double antigen immunofluorescence histochemistry for transmitter-synthesizing enzymes (glutamic acid decarboxylase and tyrosine hydroxylase) and the thymidine analogue, bromodeoxyuridine. The method permits simple, rapid, and effective anatomical detection,...
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