Article
A method for the rapid analysis of neuronal proportions and neurite morphology in primary cultures.
Experimental neurology - 1 Feb 1998
Isaacs K R, Hanbauer I, Jacobowitz D M
Abstract excerpt
This article provides basic guidelines for a rapid analysis of subpopulation proportions and neurite morphology in primary cultures. We describe, in E14 mesencephalic primary cultures, an immunohistochemical method for the simultaneous identification of multiple neuronal phenotypes and an estimat...
Topics
- Animals
- Calbindin 2
- Cell Culture Techniques
- Cells, Cultured
- Embryo, Mammalian
- Fluorescent Dyes
- Guidelines as Topic
- Immunohistochemistry
- Mesencephalon
- Nerve Tissue Proteins
- Neurites
- Neurons
- Phenotype
- Rats
- Rats, Sprague-Dawley
- Reagent Kits, Diagnostic
- Reproducibility of Results
- S100 Calcium Binding Protein G