Article
Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons.
PloS one - 1 Jan 2012
Pinto Luísa, Mateus-Pinheiro António, Morais Mónica, Bessa João Miguel, Sousa Nuno
Abstract excerpt
Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and...
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