Article
GAD and GABA in an enriched population of cultured GABAergic neurons from rat cerebral cortex.
Neurochemical research - 1 Aug 1991
Rimvall K, Martin D L
Abstract excerpt
To study various aspects of GABAergic metabolism in an easily accessible system, dissociated cells from postnatal rat cerebral cortex were cultured in a serum-based medium and characterized morphologically and biochemically. The majority (70-96%) of the neurons were GABAergic as determined by three double-labeling procedures. The specific activity of glutamine synthetase in the cultures was 4-5% of the levels in...
Topics
- Amino Acids
- Animals
- Animals, Newborn
- Biological Transport
- Blotting, Western
- Cells, Cultured
- Cerebral Cortex
- Glutamate Decarboxylase
- Glutamate-Ammonia Ligase
- Isoenzymes
- Molecular Weight