Differences in neuronal and glial cell phenotypic expression in neuron-glia cocultures: influence of glia-conditioned media and living glial cell substrata.

Abstract excerpt

Neuron-glia cocultures were prepared using, as a source for glial cells, either C6 glia (2B clone) of early (2B23) or late (2B111) passages or advanced passages of glial cells derived from primary cultures prepared from aged mouse cerebral hemispheres (MACH). Six-day-old chick embryo cerebral hemispheres (E6CH) were the source of neuron-enriched cultures. Glutamine synthetase (GS) activity was used as a marker...

Topics

• 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
• 2',3'-Cyclic-Nucleotide Phosphodiesterases
• Animals
• Cells, Cultured
• Chick Embryo
• Choline O-Acetyltransferase
• Culture Media
• Glutamate-Ammonia Ligase
• Glutamine
• Immunohistochemistry