Article
Parallel genotyping of over 10,000 SNPs using a one-primer assay on a high-density oligonucleotide array.
Genome research - 1 Mar 2004
Matsuzaki Hajime, Loi Halina, Dong Shoulian, Tsai Ya-Yu, Fang Joy, Law Jane, Di Xiaojun, Liu Wei-Min, Yang Geoffrey, Liu Guoying, Huang Jing, Kennedy Giulia C, Ryder Thomas B, Marcus Gregory A, Walsh P Sean, Shriver Mark D, Puck Jennifer M, Jones Keith W, Mei Rui
Abstract excerpt
The analysis of single nucleotide polymorphisms (SNPs) is increasingly utilized to investigate the genetic causes of complex human diseases. Here we present a high-throughput genotyping platform that uses a one-primer assay to genotype over 10,000 SNPs per individual on a single oligonucleotide array. This approach uses restriction digestion to fractionate the genome, followed by amplification of a specific...
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