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N-Terminal protein modifications in an insect cell-free protein synthesis system and their identification by mass spectrometry.

Proteomics - 1 Aug 2006

Suzuki Takashi, Ito Masaaki, Ezure Toru, Shikata Masamitsu, Ando Eiji, Utsumi Toshihiko, Tsunasawa Susumu, Nishimura Osamu

Abstract excerpt

To evaluate the ability of an insect cell-free protein synthesis system to generate proper N-terminal cotranslational protein modifications such as removal of the initiating Met, N-acetylation, and N-myristoylation, several mutants were constructed using truncated human gelsolin (tGelsolin) as a model protein. Tryptic digests of these mutants were analyzed by MALDI-TOF MS and MALDI-quadrupole-IT-TOF MS. The...

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PMID: 16835852
DOI: 10.1002/pmic.200600126

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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