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Characterization of protein variants and post-translational modifications: ESI-MSn analyses of intact proteins eluted from polyacrylamide gels.

Molecular & cellular proteomics : MCP - 1 Aug 2003

Claverol Stéphane, Burlet-Schiltz Odile, Gairin Jean Edouard, Monsarrat Bernard

Abstract excerpt

We have developed a strategy to characterize protein isoforms, resulting from single-point mutations and post-translational modifications. This strategy is based on polyacrylamide gel electrophoresis separation of protein isoforms, mass spectrometry (MS) and MSn analyses of intact proteins, and tandem MS analyses of proteolytic peptides. We extracted protein isoforms from polyacrylamide gels by passive elution...

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PMID: 12832455
DOI: 10.1074/mcp.T300003-MCP200

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