Article
A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phos-tag SDS-PAGE.
PloS one - 1 Jan 2019
Kinoshita-Kikuta Emiko, Tanikawa Ayane, Hosokawa Takuro, Kiwado Aya, Moriya Koko, Kinoshita Eiji, Koike Tohru, Utsumi Toshihiko
Abstract excerpt
To establish a strategy for identifying protein-N-myristoylation-dependent phosphorylation of cellular proteins, Phos-tag SDS-PAGE was performed on wild-type (WT) and nonmyristoylated mutant (G2A-mutant) FMNL2 and FMNL3, phosphorylated N-myristoylated model proteins expressed in HEK293 cells. The difference in the banding pattern in Phos-tag SDS-PAGE between the WT and G2A-mutant FMNL2 indicated the presence of...
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