Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.

doi:10.1128/JCM.00449-06

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Article

Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.

Journal of clinical microbiology - 1 Jul 2006

Halvas Elias K, Aldrovandi Grace M, Balfe Peter, Beck Ingrid A, Boltz Valerie F, Coffin John M, Frenkel Lisa M, Hazelwood J Darren, Johnson Victoria A, Kearney Mary, Kovacs Andrea, Kuritzkes Daniel R, Metzner Karin J, Nissley Dwight V, Nowicki Marek, Palmer Sarah, Ziermann Rainer, Zhao Richard Y, Jennings Cheryl L, Bremer James, Brambilla Don, Mellors John W

Abstract excerpt

We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant...

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Identifiers

PMID: 16825395
DOI: 10.1128/JCM.00449-06
PMCID: PMC1489464

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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