Methods for studying adaptation to the DNA damage checkpoint in yeast.

Abstract excerpt

When yeast are faced with irreparable DNA damage, they will first arrest in G2/M, via the DNA damage checkpoint pathway, but will subsequently adapt to that arrest and resume division. Here, we summarize assays that we have used to examine checkpoint adaptation. Specifically, we discuss the merits of inducing DNA damage with ionizing radiation (IR) and IR-mimetic drugs, HO, and the cdc13-1 mutation. We also...

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