Genotyping of apolipoprotein A-IV by digestion of amplified DNA with restriction endonuclease Fnu4HI: use of a tailored primer to abolish additional recognition sites during the gene amplification.

Abstract excerpt

Apolipoprotein A-IV (apoA-IV) is involved in the metabolism of chylomicrons and high density lipoproteins. It displays genetic polymorphism due to two co-dominant alleles apoA-IV1 and apoA-IV2. The mutation that causes the polymorphism is a G to T substitution in the third base of codon 360 in the apoA-IV2 allele which results in a glutamine (Gln) to histidine (His) change of amino acid 360. This substitution...

Topics

• Apolipoproteins A
• Base Sequence
• DNA
• Deoxyribonucleases, Type II Site-Specific
• Female
• Genotype
• Humans
• Male
• Molecular Sequence Data
• Pedigree
• Polymerase Chain Reaction