Article
Reporter system for the detection of in vivo gene conversion: changing colors from blue to green using GFP variants.
Molecular biotechnology - 1 Jun 2006
Sommer Jeffrey R, Alderson Jon, Laible Goetz, Petters Robert M
Abstract excerpt
We have devised a system for the study of in vivo gene correction based on the detection of color variants of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria. The intensity and spectra of the fluorescence emitted by the blue (BFP) and red-shifted (EGFP) variants of GFP differ from each other. We modified one nucleotide from an EGFP expression vector that we predicted would yield a blue...
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