Article
Low-resolution mapping of untagged mutations.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Ponce M Rosa, Robles Pedro, Lozano Francisca M, Brotóns M Asunción, Micol José L
Abstract excerpt
The mapping method detailed here is based on the multiplex polymerase chain reaction (PCR) coamplification of 32 molecular markers, using fluorescently labeled oligonucleotides as primers. For the genotyping of a single plant from a mapping population, only two simultaneous amplifications are required, the products of which are finally electrophoresed in an automated DNA sequencer controlled by fragment analysis...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.