Article
Paclitaxel-resistant human ovarian cancer cells have mutant beta-tubulins that exhibit impaired paclitaxel-driven polymerization.
The Journal of biological chemistry - 4 Jul 1997
Giannakakou P, Sackett D L, Kang Y K, Zhan Z, Buters J T, Fojo T, Poruchynsky M S
Abstract excerpt
Acquired resistance to paclitaxel can be mediated by P-glycoprotein or by alterations involving tubulin. We report two paclitaxel-resistant sublines derived from 1A9 human ovarian carcinoma cells. Single-step paclitaxel selection with verapamil yielded two clones that are resistant to paclitaxel...
Topics
- Antineoplastic Agents, Phytogenic
- Binding Sites
- Drug Resistance, Neoplasm
- Epothilones
- Epoxy Compounds
- Female
- Humans
- Models, Molecular
- Nucleic Acid Hybridization
- Ovarian Neoplasms
- Paclitaxel
- Phenotype
- Polymerase Chain Reaction
- Polymers
- Thiazoles
- Tubulin
- Tumor Cells, Cultured