Article
Conserved residues are functionally distinct within transketolases of different species.
Biochemistry - 10 Dec 1996
Singleton C K, Wang J J, Shan L, Martin P R
Abstract excerpt
Most of the amino acid residues which interact with thiamine pyrophosphate are highly conserved among enzymes which use this cofactor. The possible roles of several such residues in cofactor binding, catalysis, and/or substrate binding were examined for human transketolase. Mutations in H110 resulted in dramatic reductions to 2% or less of the normal activity. No alterations were found in the K(m)app's for the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.