Article
Suppressor mutations define two regions in the Cbp1 protein important for mitochondrial cytochrome b mRNA stability in Saccharomyces cerevisiae.
Current genetics - 1 Aug 2003
Islas-Osuna Maria A, Ellis Timothy P, Mittelmeier Telsa M, Dieckmann Carol L
Abstract excerpt
Nuclear-encoded Cbp1 stabilizes and promotes translation of mitochondrial cytochrome b (COB) mRNA. A CCG triplet within the 5'UTR of COB mRNA is essential for Cbp1-dependent stability. Like cbp1 mutations, mutation of any nucleotide in CCG results in degradation of COB transcripts. In this study, CBP1-linked pseudorevertants of the temperature-sensitive CCU strain were isolated. The suppressors are missense...
Topics
- Alleles
- Amino Acid Sequence
- Carrier Proteins
- Cytochromes b
- DNA Primers
- DNA, Mitochondrial
- Molecular Sequence Data
- Mutagenesis
- Mutation, Missense
- Plasmids
- Protein Biosynthesis