Optimization of culture and storage conditions for an in vitro system to evaluate thymocyte phenotype and function.

doi:10.1016/j.jim.2006.03.001

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Article

Optimization of culture and storage conditions for an in vitro system to evaluate thymocyte phenotype and function.

Journal of immunological methods - 30 May 2006

Young Charlene D, Angel Jonathan B

Abstract excerpt

Studies on thymopoiesis are critical to the understanding of T-cell homeostasis as well as the host response to T-cell depletion. Various in vitro culture systems have been used in the study of thymocyte development; however it is unclear if current co-culture methods have been fully optimized. In this study in vitro suspension cultures have been re-evaluated and the optimal storage conditions for thymocytes have...

Topics

Adolescent
Child
Child, Preschool
Coculture Techniques
Epithelial Cells
Humans
Infant
Interleukin-7
Phenotype
Receptors, Interleukin-7
Refrigeration
T-Lymphocyte Subsets
Thymus Gland
Tissue Preservation

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Open in PubMed

Identifiers

PMID: 16647713
DOI: 10.1016/j.jim.2006.03.001

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