Article
Isolation, cultivation and phenotypic characterization of rat thymic dendritic cells.
Thymus - 1 Jan 2000
Ilić V, Colić M, Kosec D
Abstract excerpt
Rat thymic dendritic cells (TDC) were isolated from thymic cell suspension by Nycodenz gradients of different densities and osmolarities. After cultivation of these cells for 3 days in the conditioned medium (TE-R 2.5 + HT supernatant) prepared by cocultivation of a medullary thymic epithelial cell line (TE-R 2.5) and hydrocortisone resistant thymocytes, the purity (75-85%) and survival of TDC significantly...
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