Article
Genetics of subpeptin JM4-A and subpeptin JM4-B production by Bacillus subtilis JM4.
Biochemical and biophysical research communications - 16 Jun 2006
Wu Shimei, Zhong Jin, Huan Liandong
Abstract excerpt
Subpeptin JM4-A and subpeptin JM4-B are two novel antimicrobial peptides produced by Bacillus subtilis JM4. To identify putative genes involved in their production, degenerate PCR primers targeted to conserved motifs of nonribosomal peptide synthetases (NRPSs) were used. A resulting 1.2 kb PCR product had high sequence similarity to genes of NRPSs, and then a 2.8 kb DNA fragment flanking it was cloned...
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