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Article

High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments.

Journal of neuroscience methods - 30 Sept 2006

Hagemann Carsten, Meyer Christoph, Stojic Jelena, Eicker Sven, Gerngras Stefanie, Kühnel Siglinde, Roosen Klaus, Vince Giles Hamilton

Abstract excerpt

In order to investigate the impact of signalling proteins on the phenotype and malignant behavior of glioblastoma cells, we optimized the transfection procedure of human glioblastoma cell lines U251, U373, GaMG and of primary cells obtained from a patient's tumor using nucleofection technology in conjunction with plasmid pmaxGFP. We describe the optimization procedure, show that a high percentage of the cells can...

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Identifiers

PMID: 16621008
DOI: 10.1016/j.jneumeth.2006.03.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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