Article
Transfection of wild-type and 'Fanconi anemia-like' mouse lymphoma mutant cells by electroporation.
Mutation research - 1 Jul 1991
Fraser D, Diatloff-Zito C, Moustacchi E
Abstract excerpt
An electroporation protocol for the successful transfection of mouse lymphoblastoid cells has been developed. Two cell lines, a normal and a mutant sensitive to DNA cross-linking agents, were used. The optimum conditions of electroporation in terms of uptake of the fluorescent dye lucifer yellow...
Topics
- Animals
- Blotting, Southern
- Drug Resistance
- Electric Stimulation
- Fanconi Anemia
- Fluorescent Dyes
- Genetic Techniques
- Gentamicins
- Isoquinolines
- Lymphoma
- Mice
- Mutation
- Transfection
