Article
Transfection of wild-type and 'Fanconi anemia-like' mouse lymphoma mutant cells by electroporation.
Mutation research - 1 Jul 1991
Fraser D, Diatloff-Zito C, Moustacchi E
Abstract excerpt
An electroporation protocol for the successful transfection of mouse lymphoblastoid cells has been developed. Two cell lines, a normal and a mutant sensitive to DNA cross-linking agents, were used. The optimum conditions of electroporation in terms of uptake of the fluorescent dye lucifer yellow coupled with low toxicity were established. Subsequently, these conditions were used to achieve stable transfection by...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.