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Article

Heated oligonucleotide ligation assay (HOLA): an affordable single nucleotide polymorphism assay.

Journal of medical entomology - 1 Mar 2006

Black W C, Gorrochotegui-Escalante N, Duteau N M

Abstract excerpt

Most single nucleotide polymorphism (SNP) detection requires expensive equipment and reagents. The oligonucleotide ligation assay (OLA) is an inexpensive SNP assay that detects ligation between a biotinylated "allele-specific detector" and a 3' fluorescein-labeled "reporter" oligonucleotide. No l...

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PMID: 16619605
DOI: 10.1603/0022-2585(2006)043[0238:holaha]2.0.co;2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-16619605-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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