Article
Characterization of recombinant Xenopus laevis type I iodothyronine deiodinase: substitution of a proline residue in the catalytic center by serine (Pro132Ser) restores sensitivity to 6-propyl-2-thiouracil.
Endocrinology - 1 Jul 2006
Kuiper George G J M, Klootwijk Willem, Morvan Dubois Ghislaine, Destree Olivier, Darras Veerle M, Van der Geyten Serge, Demeneix Barbara, Visser Theo J
Abstract excerpt
In frogs such as Rana and Xenopus, metamorphosis does not occur in the absence of a functional thyroid gland. Previous studies indicated that coordinated development in frogs requires tissue and stage-dependent type II and type III iodothyronine deiodinase expression patterns to obtain requisite levels of intracellular T(3) in tissues at the appropriate stages of metamorphosis. No type I iodothyronine deiodinase...
Topics
- 3' Untranslated Regions
- Amino Acid Sequence
- Animals
- Base Sequence
- Catalytic Domain
- Iodide Peroxidase
- Kinetics
- Molecular Sequence Data
- Mutation
- Proline