Article
Phenylethynylpyrene-labeled oligonucleotide probes for excimer fluorescence SNP analysis of 23S rRNA gene in clarithromycin-resistant Helicobacter pylori strains.
Mutation research - 25 Jul 2006
Prokhorenko Igor A, Malakhov Andrei D, Kozlova Anna A, Momynaliev Kuvat, Govorun Vadim M, Korshun Vladimir A
Abstract excerpt
The use of phenylethynylpyrene excimer forming pair in the design of specific fluorescent probes for determination of A2144G (A2143G and/or A2143C) mutations in 23S rRNA gene of Helicobacter pylori is described. Analysis of fluorescence spectra of model duplexes revealed optimal positions of fluorophore residues in the probe sequences for maximum efficiency of SNP detection. Application of excimer forming probes...
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