Article
[Proliferation of natural killer T cells in umbilical cord blood and peripheral blood and their different phenotypes].
Zhongguo shi yan xue ye xue za zhi - 1 Feb 2006
Liu Yan, Fan Hua-Hua, Ruan Ming, Gao Li, Nie Xiao-Xuan, Yang Yi-Ming, Lu Hua-Zhong, Gao Feng
Abstract excerpt
Purpose of this study was to establish an effective method in vitro to proliferate natural killer T (NKT) cells from umbilical cord blood (UCB) and peripheral blood (PB), and to study their different phenotype. Mononuclear cells (MNC) from UCB and PB were cultured in the presence of IL-2 (100 U/ml), with or without alpha-Galcer. TCR Valpha24 Vbeta11 double positive natural killer T-cells (NKT cells) and their...
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