Article
[Molecular biological study of the vaccinia virus genome. IV. The late nonstructural 36K protein of vaccinia virus is vitally important].
Molekuliarnaia biologiia - 1 Jan 2000
Shchelkunov S N, Riazankina O I, Gashnikov P B, Totmenin A B, Chizhikov V E, Malygin E G
Abstract excerpt
Two genetics markers: the herpes simplex virus thymidine kinase and Escherichia coli beta-galactosidase genes were inserted into the 36K protein gene of vaccinia virus located in a HindIII-P DNA fragment. An unstability of recombinant viruses with Lac(+)-phenotype were discovered. A mechanism of viruses unstable variants formation was proposed, it was confirmed by the results of hybridisation analyses of virus...
Topics
- Autoradiography
- Cloning, Molecular
- Escherichia coli
- Genes, Viral
- Genetic Markers
- Molecular Weight
- Nucleic Acid Hybridization
- Phenotype
- Plasmids
- Recombination, Genetic