Article
[Molecular biological study of the vaccinia virus genome. IV. The late nonstructural 36K protein of vaccinia virus is vitally important].
Molekuliarnaia biologiia - 1 Jan 2000
Shchelkunov S N, Riazankina O I, Gashnikov P B, Totmenin A B, Chizhikov V E, Malygin E G
Abstract excerpt
Two genetics markers: the herpes simplex virus thymidine kinase and Escherichia coli beta-galactosidase genes were inserted into the 36K protein gene of vaccinia virus located in a HindIII-P DNA fragment. An unstability of recombinant viruses with Lac(+)-phenotype were discovered. A mechanism of viruses unstable variants formation was proposed, it was confirmed by the results of hybridisation analyses of virus...
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