Article
Complementation of a vesicular stomatitis virus glycoprotein G mutant with wild-type protein expressed from either a bovine papilloma virus or a vaccinia virus vector system.
Virology - 1 Oct 1990
Lefkowitz E J, Pattnaik A K, Ball L A
Abstract excerpt
Using a complementation assay, we have evaluated the potential of two eukaryotic expression systems to produce functional virus proteins. The first expression system was based on a bovine papilloma virus (BPV) eukaryotic expression vector which contained a copy of the gene for the membrane glycop...
Topics
- Animals
- Bovine papillomavirus 1
- Cattle
- DNA-Directed RNA Polymerases
- Gene Expression
- Genetic Complementation Test
- HeLa Cells
- Humans
- Mice
- Mice, Inbred Strains
- Mutation
- Plasmids
- Platelet Membrane Glycoproteins
- Promoter Regions, Genetic
- RNA, Messenger
- Temperature
- Thrombospondins
- Transformation, Genetic