Article
Evidence of the capability of the CMV enhancer to activate in trans gene expression in mammalian cells.
DNA and cell biology - 1 Mar 2006
D'Aiuto Leonardo, De Marco Richard, Edward Nwanegbo, Rizzo Annalisa, Chaillet J Richard, Montecalvo Angela, Lotze Michael T, Gambotto Andrea
Abstract excerpt
Studies have reported that an enhancer can act in trans when artificially, noncovalently bridged to the promoter by a protein-linked biotin:streptavidin complex, or when an enhancer and a promoter are located on separate concatenated plasmids. To investigate such transactivation in mammalian cells, we constructed CMV promoter-enhancer mutants driving the expression of the EGFP reporter gene and transfected...
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