Molecular characterization of Saccharomyces cerevisiae URA6 gene. DNA sequence, mutagenesis analysis, and cell cycle regulation relevant to its suppression mechanism to cdc8 mutation.

Abstract excerpt

Yeast SOC8 DNA fragment was isolated as a wild type dominant suppressor of cdc8 mutation. We have used Bal31 deletion analysis to define the minimal 1 kilobase HpaI-NcoI DNA element required for complementing the cdc8 mutation. The complementing sequence harbored a multicopy plasmid also enhanced by uridine monophosphate kinase in crude extracts. DNA sequence analysis revealed an open reading frame encoding a...

Topics

• Amino Acid Sequence
• Base Sequence
• Blotting, Northern
• Cell Cycle
• Genes, Fungal
• Molecular Sequence Data
• Mutagenesis
• Mutation
• Nucleoside-Phosphate Kinase
• Plasmids