Article
Genetic analysis of the gyrase A-like domain of DNA topoisomerase II of Saccharomyces cerevisiae.
Genetics - 1 Aug 1991
Thomas W, Spell R M, Ming M E, Holm C
Abstract excerpt
We have undertaken a genetic analysis of heat-sensitive and cold-sensitive mutations in TOP2, the gene encoding yeast DNA topoisomerase II. Deletion mapping was used to localize 14 heat-sensitive and four cold-sensitive top2 mutations created by a method biased toward mutations in the 3' two-thir...
Topics
- Base Sequence
- DNA Mutational Analysis
- DNA Topoisomerases, Type II
- Genes, Fungal
- Genes, Suppressor
- Genetic Complementation Test
- Molecular Sequence Data
- Phenotype
- Protein Conformation
- Saccharomyces cerevisiae
- Transformation, Genetic