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Article

Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.

RNA (New York, N.Y.) - 1 May 2006

Brenner Tamara J, Guthrie Christine

Abstract excerpt

Snu114 is a U5 snRNP protein essential for pre-mRNA splicing. Based on its homology with the ribosomal translocase EF-G, it is thought that GTP hydrolysis by Snu114 induces conformational rearrangements in the spliceosome. We recently identified allele-specific genetic interactions between SNU114 and genes encoding three other U5 snRNP components, Prp8 and two RNA-dependent ATPases, Prp28 and Brr2, required for...

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Identifiers

PMID: 16540695
DOI: 10.1261/rna.2319806
PMCID: PMC1440915

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.PMID 16540695Read ↗

Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation.Semantic proximity Read ↗

The ribosomal translocase homologue Snu114p is involved in unwinding U4/U6 RNA during activation of the spliceosome.Semantic proximity Read ↗

The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.Semantic proximity Read ↗

Mutagenesis suggests several roles of Snu114p in pre-mRNA splicing.Semantic proximity Read ↗

The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase.Semantic proximity Read ↗

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.Semantic proximity Read ↗

Analysis of synthetic lethality reveals genetic interactions between the GTPase Snu114p and snRNAs in the catalytic core of the Saccharomyces cerevisiae spliceosome.Semantic proximity Read ↗

Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.Semantic proximity Read ↗

Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.Semantic proximity Read ↗

A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity.Semantic proximity Read ↗

ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8.Semantic proximity Read ↗

Allele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.Semantic proximity Read ↗

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