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Article

Genotyping the HFE gene by melting point analysis with the LightCycler system: Pros and cons.

Blood cells, molecules & diseases - 1 Jan 2000

Bach V, Barceló M J, Altés A, Remacha A, Félez J, Baiget M

Abstract excerpt

The assay that combines rapid-cycle PCR with allele-specific fluorescent probe melting profiles performed on the Roche Diagnostics LightCycler is commonly employed for genotyping the HFE gene. We report three illustrative cases of the pros and cons of this method. In two cases, atypical melting curves allows the identification of new DNA substitutions in the HFE gene, whereas, in the third case, a typical melting...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 16515868
DOI: 10.1016/j.bcmd.2005.12.030

Genes

HFE

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Genotyping the HFE gene by melting point analysis with the LightCycler system: Pros and cons.PMID 16515868Read ↗

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