Article
Rapid genotyping of human platelet antigen 5 with fluorophore-labelled hybridization probes on the LightCycler.
British journal of haematology - 1 Aug 2001
Kretzschmar E, Von Ahsen N, Trobisch H
Abstract excerpt
Genotyping of human platelet antigens (HPAs) can be useful for the diagnosis and therapy of alloimmune thrombocytopenic syndromes such as neonatal alloimmune thrombocytopenic purpura, post-transfusion purpura and refractoriness to platelet transfusion therapy. We developed a single-tube method for HPA-5 genotyping on the LightCycler that combines rapid-cycle polymerase chain reaction with allele-specific...
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