Article
Opsin is present as dimers in COS1 cells: identification of amino acids at the dimeric interface.
Proceedings of the National Academy of Sciences of the United States of America - 28 Feb 2006
Kota Parvathi, Reeves Philip J, Rajbhandary Uttam L, Khorana H Gobind
Abstract excerpt
Rhodopsin in the disk membranes of rod outer segments serves as the dim-light photoreceptor and is a prototypic member of a G protein-coupled receptor family. Electron and atomic-force microscopy indicate that rhodopsin is present as dimers in the native membranes. Here, we have expressed the protein, opsin, in COS1 cells and have studied its molecular state by using FRET and by intermolecular cross-linking after...
Topics
- Amino Acid Sequence
- Amino Acids
- Animals
- Binding Sites
- COS Cells
- Cell Membrane
- Chlorocebus aethiops
- Cross-Linking Reagents
- Cysteine
- Dimerization
- Fluorescence Resonance Energy Transfer
- Molecular Sequence Data
- Mutation
- Phenanthrolines