Structure and function in rhodopsin. Separation and characterization of the correctly folded and misfolded opsins produced on expression of an opsin mutant gene containing only the native intradiscal cysteine codons.

Abstract excerpt

Previous mutagenesis studies have indicated the requirement of a tertiary structure in the intradiscal region with a disulfide bond between Cys-110 and Cys-187 for the correct assembly and/or function of rhodopsin. We have now studied a rhodopsin mutant in which only the natural intradiscal cysteines at positions 110, 185, and 187 are present while all the remaining seven cysteines in the wild-type bovine...

Topics

• Amino Acid Sequence
• Animals
• Binding Sites
• Cattle
• Cell Line
• Circular Dichroism
• Codon
• Cysteine
• Gene Expression
• Molecular Sequence Data
• Molecular Structure