Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Use of Rab GTPases to study lipid trafficking in normal and sphingolipid storage disease fibroblasts.

Methods in enzymology - 1 Jan 2005

Choudhury Amit, Marks David L, Pagano Richard E

Abstract excerpt

We describe methods for studying lipid transport in normal and sphingolipid storage disease fibroblasts. These techniques include endocytic assays with fluorescent sphingolipid analogs, expression of dominant negative (DN) Rab GTPases, and methods of manipulating cholesterol levels in intact cells and isolated cell membranes. These methods should be useful in future studies of lipid trafficking in normal and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biological Transport
Share to Cholesterol
Share to Glutathione Transferase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Golgi Apparatus

Share to Guanine Nucleotide Dissociation Inhibitors

Share to Lipid Metabolism

Share to rab GTP-Binding Proteins

Source

Identifiers

PMID: 16473585
DOI: 10.1016/S0076-6879(05)03014-4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Use of Rab GTPases to study lipid trafficking in normal and sphingolipid storage disease fibroblasts.PMID 16473585Read ↗

Cholesterol trapping in Niemann-Pick disease type B fibroblasts can be relieved by expressing the phosphotyrosine binding domain of GULP.Semantic proximity Read ↗

Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements.Semantic proximity Read ↗

1-Deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathwaySemantic proximity Read ↗

Modulation of cellular cholesterol transport and homeostasis by Rab11.Semantic proximity Read ↗

Cholesterol accumulation sequesters Rab9 and disrupts late endosome function in NPC1-deficient cells.Semantic proximity Read ↗

Raft partitioning of the yeast uracil permease during trafficking along the endocytic pathway.Semantic proximity Read ↗

Accumulation of sphingolipids in SAP-precursor (prosaposin)-deficient fibroblasts occurs as intralysosomal membrane structures and can be completely reversed by treatment with human SAP-precursor.Semantic proximity Read ↗

Alterations in the properties of the cell membrane due to glycosphingolipid accumulation in a model of Gaucher disease.Semantic proximity Read ↗

Lipid membrane domains in cell surface and vacuolar systems.Semantic proximity Read ↗

Clathrin-mediated endocytosis is impaired in type A-B Niemann-Pick disease model cells and can be restored by ICAM-1-mediated enzyme replacement.Semantic proximity Read ↗

Analysis of a Chinese hamster ovary cell mutant with defective mobilization of cholesterol from the plasma membrane to the endoplasmic reticulum.Semantic proximity Read ↗

The End2 mutation in CHO cells slows the exit of transferrin receptors from the recycling compartment but bulk membrane recycling is unaffected.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.