Article
A new protein superfamily includes two novel 3-methyladenine DNA glycosylases from Bacillus cereus, AlkC and AlkD.
Molecular microbiology - 1 Mar 2006
Alseth Ingrun, Rognes Torbjørn, Lindbäck Toril, Solberg Inger, Robertsen Kristin, Kristiansen Knut Ivan, Mainieri Davide, Lillehagen Lucy, Kolstø Anne-Brit, Bjørås Magnar
Abstract excerpt
Soil bacteria are heavily exposed to environmental methylating agents such as methylchloride and may have special requirements for repair of alkylation damage on DNA. We have used functional complementation of an Escherichia coli tag alkA mutant to screen for 3-methyladenine DNA glycosylase genes in genomic libraries of the soil bacterium Bacillus cereus. Three genes were recovered: alkC, alkD and alkE. The amino...
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