Article
Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: genotyping by MADGE.
Clinical biochemistry - 1 Jun 2006
Zivković Maja, Raković Aleksandar, Stanković Aleksandra
Abstract excerpt
OBJECTIVES: The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. DESIGN AND METHODS: Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. RESULTS: PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. CONCLUSIONS: We designed a faster,...
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