A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.

doi:10.1152/physiolgenomics.1999.1.2.71

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Article

A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.

Physiological genomics - 31 Aug 1999

Hunt C C, Burley J E, Chapman C M, Beilby J P

Abstract excerpt

We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT(1)) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I...

Topics

Alleles
DNA
DNA Primers
Electrophoresis, Polyacrylamide Gel
Genotype
Humans
Mutagenesis
Nucleic Acid Hybridization
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
Receptors, Angiotensin
Reproducibility of Results

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Open in PubMed

Identifiers

PMID: 11015563
DOI: 10.1152/physiolgenomics.1999.1.2.71

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