Article
A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism.
Physiological genomics - 31 Aug 1999
Hunt C C, Burley J E, Chapman C M, Beilby J P
Abstract excerpt
We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT(1)) A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I...
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