Article
High-throughput assay of DNA methylation based on methylation-specific primer and SAGE.
Biochemical and biophysical research communications - 17 Mar 2006
Wang Xianliang, Zhang Chi, Zhang Lijun, Wang Xiaoli, Xu Shunqing
Abstract excerpt
Mapping of genomic DNA methylation is a dispensable part of functional genome. We have developed a novel method based on methylation-specific primer and serial analysis of gene expression, called MSP-SAGE, with potential of high-throughput quantification of genomic DNA methylation. We used a 6-mer methylation-specific primer to extend the methylated CpG sequences other than non-methylated CpG sequences. The 17 bp...
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