Molecular cloning of the gene encoding Vibrio metalloproteinase vimelysin and isolation of a mutant with high stability in organic solvents.

doi:10.1093/jb/mvi173

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Article

Molecular cloning of the gene encoding Vibrio metalloproteinase vimelysin and isolation of a mutant with high stability in organic solvents.

Journal of biochemistry - 1 Dec 2005

Takahashi Toshihiro, Ng Kenneth K-S, Oyama Hiroshi, Oda Kohei

Abstract excerpt

Vimelysin is a unique metalloproteinase from Vibrio sp. T1800 exhibiting high activity at low temperature and high stability in organic solvents such as ethanol. A 1,821 bp open reading frame of the vimelysin gene encoded 607 amino acid residues consisting of an N-terminal pro-region, a mature enzyme, and a C-terminal pro-region. The mature enzyme region showed 80%, 57% and 35% sequence identity with the mature...

Topics

Bacterial Proteins
Cloning, Molecular
Genes, Bacterial
Metalloendopeptidases
Metalloproteases
Molecular Sequence Data
Mutation
Protein Stability
Solvents
Vibrio

Source

Open in PubMed

Identifiers

PMID: 16428299
DOI: 10.1093/jb/mvi173

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