Article
Cloning and characterization of a metalloprotease from Vibrio harveyi strain AP6.
Gene - 16 Jan 2003
Teo Jeanette W P, Zhang Lian Hui, Poh Chit Laa
Abstract excerpt
A metalloprotease gene pap6 was cloned from Vibrio harveyi strain AP6. Sequence analysis showed that pap6 was 2034 bp in length and predicted to encode a peptide of 677 amino acids with a molecular mass of 75 kDa. SDS-PAGE analysis of the purified Pap6 revealed that it was 35 kDa in size. N-terminal amino acid sequencing established that the mature protein began at Leu-191, suggesting that the preprotein of Pap6...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Hydrogen-Ion Concentration
- Metalloendopeptidases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation