Engineering cotton (+)-delta-cadinene synthase to an altered function: germacrene D-4-ol synthase.

Abstract excerpt

The combined approaches of rational design and random mutagenesis were applied to generate a sesquiterpene synthase with an altered activity. Due to the lack of a convenient screen for sesquiterpene synthase activity, a high-throughput dual-activity screen was used by fusing (+)-delta-cadinene synthase to chloramphenicol acetyltransferase (CAT). The gene encoding (+)-delta-cadinene synthase was mutagenized using...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alkyl and Aryl Transferases
• Share to Chloramphenicol O-Acetyltransferase