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Article

Directed evolution to generate cycloartenol synthase mutants that produce lanosterol.

Organic letters - 18 Apr 2002

Meyer Michelle M, Xu Ran, Matsuda Seiichi P T

Abstract excerpt

Cycloartenol synthase converts oxidosqualene to cycloartenol, a pentacyclic isomer of the animal and fungal sterol precursor lanosterol. We used directed evolution to find cycloartenol synthase residues that affect cyclopropyl ring formation, selecting randomly generated cycloartenol synthase mutants for their ability to genetically complement a yeast strain lacking lanosterol synthase. To increase the likelihood...

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PMID: 11950371
DOI: 10.1021/ol0257225

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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