Article
The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions.
Nucleic acids research - 25 Jul 1992
Xiao W, Samson L
Abstract excerpt
We previously cloned a yeast DNA fragment that, when fused with the bacterial lacZ promoter, produced O6-methylguanine DNA repair methyltransferase (MGT1) activity and alkylation resistance in Escherichia coli (Xiao et al., EMBO J. 10,2179). Here we describe the isolation of the entire MGT1 gene and its promoter by sequence directed chromosome integration and walking. The MGT1 promoter was fused to a lacZ...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Repair
- Escherichia coli
- Gene Expression Regulation, Fungal
- Methylnitronitrosoguanidine
- Methyltransferases
- Molecular Sequence Data
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase