Article
Hammerhead ribozyme specifically inhibits mutant K-ras mRNA of human pancreatic cancer cells.
Biochemical and biophysical research communications - 18 Dec 1998
Tsuchida T, Kijima H, Oshika Y, Tokunaga T, Abe Y, Yamazaki H, Tamaoki N, Ueyama Y, Scanlon K J, Nakamura M
Abstract excerpt
We have evaluated the efficacy of an anti-K-ras hammerhead ribozyme targeted against GUU-mutated codon 12 of the K-ras gene in a cell-free system as well as in cultured pancreatic carcinoma cell lines. In the cell-free system, the anti-K-ras ribozyme specifically cleaved K-ras RNA with GUU-mutati...
Topics
- Adenocarcinoma
- Carcinoma
- Cell-Free System
- Down-Regulation
- Genes, ras
- Growth Inhibitors
- Humans
- Hydrolysis
- Mutagenesis, Site-Directed
- Pancreatic Neoplasms
- Phenotype
- RNA, Catalytic
- RNA, Messenger
- Tumor Cells, Cultured