Article
A catalytically-impaired class A beta-lactamase: 2 A crystal structure and kinetics of the Bacillus licheniformis E166A mutant.
Protein engineering - 1 Jan 1993
Knox J R, Moews P C, Escobar W A, Fink A L
Abstract excerpt
In the beta-lactamase (penicillinase) of Bacillus licheniformis 749/C, site specific mutation of Glu166 to Ala caused a million-fold reduction of catalytic activity towards both penicillin and cephalosporin substrates and resulted in the stoichiometric accumulation of the acyl enzyme. The rate of...
Topics
- Bacillus
- Binding Sites
- Cephalosporins
- Isomerism
- Kinetics
- Models, Molecular
- Mutation
- Penicillinase
- Penicillins
- Proline
- Protein Conformation
- Protein Folding
- Protein Structure, Tertiary
- Structure-Activity Relationship
- Substrate Specificity
- Water
- X-Ray Diffraction