Article
Ecological behavior of Lactobacillus reuteri 100-23 is affected by mutation of the luxS gene.
Applied and environmental microbiology - 1 Dec 2005
Tannock Gerald W, Ghazally Salina, Walter Jens, Loach Diane, Brooks Heather, Cook Gregory, Surette Michael, Simmers Cameron, Bremer Phil, Dal Bello Fabio, Hertel Christian
Abstract excerpt
The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of the luxS mutation by a plasmid construct containing luxS restored AI-2 and AI-3 synthesis. In...
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