Article
Use of minigene systems to dissect alternative splicing elements.
Methods (San Diego, Calif.) - 1 Dec 2005
Cooper Thomas A
Abstract excerpt
Pre-mRNA splicing is an essential step for gene expression in higher eukaryotes. The splicing efficiency of individual exons is determined by multiple features involving gene architecture, a variety of cis-acting elements within the exons and flanking introns, and interactions with components of the basal splicing machinery (called the spliceosome) and auxiliary regulatory factors which transiently co-assemble...
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